Extraction and Evaluation of Active Ingredients of Saussurea costus Roots and Determination of its Antibacterial Activity

Abstract Saussurea costus (Sc) plant belongs to the Asteraceae family. It contains various bioactive compounds used traditionally in treating multiple diseases. This study intends extract and evaluate active ingredients of Sc roots ( Scrs ) determine their antibacterial activities. S. were purchased from herbal market chemical compositions, including protein, lipid, carbohydrates, moisture, ash, determined according AOAC procedures 2016 with some modifications. HPLC amino acids analyzer phenolic acid composition (ScrsE) . The activities done Kirby-Bauer disc diffusion method. revealed a fusiform (or) conical tapering appearance, collapsing centre longitudinal wrinkles revealing 11-19 cm 1-2.5 for long wide, respectively. extraction yield was 9.52% weight concentrated crude 10 g. According Proximate analysis, showed 3.88% 20.12% moisture ash contents, compositions fat 75.25%, 2.51%, 1.85%, analysis that Extract contained two flavonoids. Moreover, twelve first time extract. Various seen maximum inhibition zone Streptococcus pyogenes & E.coli; minimum Bacillus subtilis Pseudomonas aeruginosa In conclusion, this investigated contents acids, fat, addition its authors recommend more future studies investigate therapeutic